human intestine epithelial cell line ls180 (ATCC)

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ATCC human intestine epithelial cell line ls180

The effects of CBD on PXR activity in human intestinal <t>LS180</t> cells using transfection assay. (A, C) Human LS180 intestinal cells were transfected with full-length hPXR plasmid together with hPXR reporter CYP3A4-luc and β-galactosidase (β-gal) control plasmids. Cells were then treated with CBD (A) at the indicated concentrations or (C) at the doses from 0.2 to 20 µM for 24 hours (n=3). (B) Human LS180 cells were transfected with full-length mPXR plasmid and mPXR reporter (CYP3A2) 3 -luc together with β-gal control plasmids. Cells were then treated with CBD at the indicated concentrations for 24 hours (n=3). Reporter gene activity was normalized to the β-gal transfection controls and the results were normalized to Relative Light Unit (RLU) per OD 420 β-gal per minute to facilitate comparisons between plates. Fold activation was calculated relative to vehicle DMSO controls. Error bars represent ± SEM.

Human Intestine Epithelial Cell Line Ls180, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intestinal+epithelial+cell+line+ls180/LS+180/pmc11217338-47-9-15
Average 96 stars, based on 577 article reviews

human intestine epithelial cell line ls180 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Cannabidiol promotes intestinal cholesterol uptake mediated by Pregnane X receptor"

Article Title: Cannabidiol promotes intestinal cholesterol uptake mediated by Pregnane X receptor

Journal: Frontiers in Endocrinology

doi: 10.3389/fendo.2024.1398462

The effects of CBD on PXR activity in human intestinal LS180 cells using transfection assay. (A, C) Human LS180 intestinal cells were transfected with full-length hPXR plasmid together with hPXR reporter CYP3A4-luc and β-galactosidase (β-gal) control plasmids. Cells were then treated with CBD (A) at the indicated concentrations or (C) at the doses from 0.2 to 20 µM for 24 hours (n=3). (B) Human LS180 cells were transfected with full-length mPXR plasmid and mPXR reporter (CYP3A2) 3 -luc together with β-gal control plasmids. Cells were then treated with CBD at the indicated concentrations for 24 hours (n=3). Reporter gene activity was normalized to the β-gal transfection controls and the results were normalized to Relative Light Unit (RLU) per OD 420 β-gal per minute to facilitate comparisons between plates. Fold activation was calculated relative to vehicle DMSO controls. Error bars represent ± SEM.

Figure Legend Snippet: The effects of CBD on PXR activity in human intestinal LS180 cells using transfection assay. (A, C) Human LS180 intestinal cells were transfected with full-length hPXR plasmid together with hPXR reporter CYP3A4-luc and β-galactosidase (β-gal) control plasmids. Cells were then treated with CBD (A) at the indicated concentrations or (C) at the doses from 0.2 to 20 µM for 24 hours (n=3). (B) Human LS180 cells were transfected with full-length mPXR plasmid and mPXR reporter (CYP3A2) 3 -luc together with β-gal control plasmids. Cells were then treated with CBD at the indicated concentrations for 24 hours (n=3). Reporter gene activity was normalized to the β-gal transfection controls and the results were normalized to Relative Light Unit (RLU) per OD 420 β-gal per minute to facilitate comparisons between plates. Fold activation was calculated relative to vehicle DMSO controls. Error bars represent ± SEM.

Techniques Used: Activity Assay, Transfection, Plasmid Preparation, Control, Activation Assay

The impacts of CBD exposure on cholesterol uptake by human intestinal LS180 cells. Human LS180 cells were treated with vehicle control or 10 µM of CBD in the presence/absence of PXR antagonist RES (50 µM) in serum-free culture media for 24 hours. (A, B) Intestinal expression of PXR target genes was measured by QPCR (n=3, two-way ANOVA, Bonferroni multiple comparisons test for multiple comparisons, *P<0.05, **P<0.01 and ***P<0.001). (C) GFP-tagged cholesterol was used to cotreat the cells for 24 hours. The degree of cholesterol uptake was quantified at Ex/Em = 485/535 nm. The result was displayed as the fold activation of relative fluorescence units compared to DMSO vehicle (n=3, two-way ANOVA, Bonferroni multiple comparisons test for multiple comparisons, **P<0.01 and ***P<0.001). (D-F) The gene expression of key intestinal cholesterol transporters was analyzed by QPCR (n=3, two-way ANOVA, Bonferroni multiple comparisons test for multiple comparisons, *P<0.05 and **P<0.01). Error bars represent ± SEM.

Figure Legend Snippet: The impacts of CBD exposure on cholesterol uptake by human intestinal LS180 cells. Human LS180 cells were treated with vehicle control or 10 µM of CBD in the presence/absence of PXR antagonist RES (50 µM) in serum-free culture media for 24 hours. (A, B) Intestinal expression of PXR target genes was measured by QPCR (n=3, two-way ANOVA, Bonferroni multiple comparisons test for multiple comparisons, *P<0.05, **P<0.01 and ***P<0.001). (C) GFP-tagged cholesterol was used to cotreat the cells for 24 hours. The degree of cholesterol uptake was quantified at Ex/Em = 485/535 nm. The result was displayed as the fold activation of relative fluorescence units compared to DMSO vehicle (n=3, two-way ANOVA, Bonferroni multiple comparisons test for multiple comparisons, **P<0.01 and ***P<0.001). (D-F) The gene expression of key intestinal cholesterol transporters was analyzed by QPCR (n=3, two-way ANOVA, Bonferroni multiple comparisons test for multiple comparisons, *P<0.05 and **P<0.01). Error bars represent ± SEM.

Techniques Used: Control, Expressing, Activation Assay, Fluorescence, Gene Expression

Derivative Assay:

Article Title: Serine 350 of Human Pregnane X Receptor Is Crucial for Its Heterodimerization with Retinoid X Receptor Alpha and Transactivation of Target Genes in Vitro and in Vivo
Article Snippet: .. The human liver carcinoma cell line HepG2, the human intestinal epithelial cell line LS180 (derived from colorectal adenocarcinoma), and the 293T cell line were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in modified Eagle’s minimal essential medium (MEM) from ATCC with 10% FBS (Hyclone, Logan, UT), 2 mM L-glutamine (Invitrogen, Carlsbad, CA), and 100 U/ml penicillin/streptomycin (Invitrogen) at 37°C in a humidified 5% CO 2 atmosphere. .. Cells were co-transfected with pGL3- CYP3A4 -luc and with pcDNA3, pcDNA3-hPXR, pcDNA3-hPXR S350D , or pcDNA3-hPXR S350A by using FuGENE 6 (Roche Diagnostics, Indianapolis, IN).

Article Title: Serine 350 of Human Pregnane X Receptor Is Crucial for Its Heterodimerization with Retinoid X Receptor Alpha and Transactivation of Target Genes in Vitro and in Vivo
Article Snippet: .. 2.2 Cell Culture The human liver carcinoma cell line HepG2, the human intestinal epithelial cell line LS180 (derived from colorectal adenocarcinoma), and the 293T cell line were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in modified Eagle’s minimal essential medium (MEM) from ATCC with 10% FBS (Hyclone, Logan, UT), 2 mM L-glutamine (Invitrogen, Carlsbad, CA), and 100 U/ml penicillin/streptomycin (Invitrogen) at 37°C in a humidified 5% CO 2 atmosphere. .. 2.3 hPXR Transactivation Assay Cells were co-transfected with pGL3- CYP3A4 -luc and with pcDNA3, pcDNA3-hPXR, pcDNA3-hPXR S350D , or pcDNA3-hPXR S350A by using FuGENE 6 (Roche Diagnostics, Indianapolis, IN).

Modification:

Article Title: Serine 350 of Human Pregnane X Receptor Is Crucial for Its Heterodimerization with Retinoid X Receptor Alpha and Transactivation of Target Genes in Vitro and in Vivo
Article Snippet: .. 2.2 Cell Culture The human liver carcinoma cell line HepG2, the human intestinal epithelial cell line LS180 (derived from colorectal adenocarcinoma), and the 293T cell line were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in modified Eagle’s minimal essential medium (MEM) from ATCC with 10% FBS (Hyclone, Logan, UT), 2 mM L-glutamine (Invitrogen, Carlsbad, CA), and 100 U/ml penicillin/streptomycin (Invitrogen) at 37°C in a humidified 5% CO 2 atmosphere. .. 2.3 hPXR Transactivation Assay Cells were co-transfected with pGL3- CYP3A4 -luc and with pcDNA3, pcDNA3-hPXR, pcDNA3-hPXR S350D , or pcDNA3-hPXR S350A by using FuGENE 6 (Roche Diagnostics, Indianapolis, IN).

Cell Culture:

Article Title: Serine 350 of Human Pregnane X Receptor Is Crucial for Its Heterodimerization with Retinoid X Receptor Alpha and Transactivation of Target Genes in Vitro and in Vivo
Article Snippet: .. 2.2 Cell Culture The human liver carcinoma cell line HepG2, the human intestinal epithelial cell line LS180 (derived from colorectal adenocarcinoma), and the 293T cell line were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and maintained in modified Eagle’s minimal essential medium (MEM) from ATCC with 10% FBS (Hyclone, Logan, UT), 2 mM L-glutamine (Invitrogen, Carlsbad, CA), and 100 U/ml penicillin/streptomycin (Invitrogen) at 37°C in a humidified 5% CO 2 atmosphere. .. 2.3 hPXR Transactivation Assay Cells were co-transfected with pGL3- CYP3A4 -luc and with pcDNA3, pcDNA3-hPXR, pcDNA3-hPXR S350D , or pcDNA3-hPXR S350A by using FuGENE 6 (Roche Diagnostics, Indianapolis, IN).

Article Title: Mutual repression between steroid and xenobiotic receptor and NF-?B signaling pathways links xenobiotic metabolism and inflammation
Article Snippet: .. The human hepatic cell line HepG2, intestinal epithelial cell line LS180, and mouse macrophage cell line RAW264.7 were obtained from ATCC and cultured in DMEM containing 10% FBS at 37°C in 5% CO 2 . ..

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